卵巢肿瘤干细胞中趋化因子的表达与细胞侵袭转移能力的关系(1)
[摘要] 目的 探讨卵巢肿瘤干细胞中趋化因子的表达与细胞侵袭转移能力的关系，为卵巢肿瘤的治疗提供新的研究思路和药物作用靶点。 方法 采用流式细胞术与RT-PCR方法检测卵巢肿瘤干细胞系CAOV-3中CXCR4的表达，通过体外微孔隔离室(Transwell)检测CXCR4对CAOV-3细胞侵袭转移能力的影响。 结果 CXCR4在CAOV-3中细胞呈阳性表达，CXCL12可促进CAOV-3细胞的迁移，CXCR4的封闭能抑制CXCL12对CAOV-3迁移的促进作用。 结论 CXCL12-CXCR4相互作用可促进卵巢肿瘤干细胞侵袭转移，在卵巢肿瘤的生长和侵袭转移过程中起着重要作用。
[关键词] 卵巢肿瘤干细胞；CXCL12；CXCR4；CAOV-3；侵袭转移
[中图分类号] R737.31 [文献标识码] A [文章编号] 1674-4721(2012)06(a)-0010-03
The relation of chemokine expression and cell invasion and metastasis in the ovarian cancer stem cells
LI Qing1,2 WANG Yan2 ZHANG Xi2 ZHU Huifen2 TANG Liangdan1
1.Department of Obstertrics and Gynecology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; 2.Department of Obstertrics and Gynecology, the Affiliated Anqing Hospital of Anhui Medical University, Anqing 246000, China
[Abstract] Objective To investigate the ovarian tumor stem cells chemokine expression and cell invasion and metastasis ability of relation, for the treatment of ovarian tumors to provide new research ideas and targets of drug action. Methods Expression of CXCR4 in ovarian cancer cellline CAOV-3 was detected by reverse transcriptase—polymerase chain reaction(RT-PCR) and flow cytometry; Transwell was used to analyze the invasion and metastasis of CAOV-3 cells in presence of CXCL12 or when CXCR4 was blocked by anti-CXCR4. Results CXCR4 in CAOV-3 cells showed positive expression, CXCL12 induced the invasion and metastasis of CAOV-3 cells，which could be effectively blocked by anti-CXCR4. Conclusion CXCL12-CXCR4 interactions may promote ovarian tumor stem cells invasion and metastasis, in ovarian tumor growth and metastasis plays an important role in the process.
[Key words] Ovarian cancer stem cells; CXCL12; CXCR4; CAOV-3; Invasion and metastasis
随着社会经济的发展与环境的各种影响，当前我国卵巢肿瘤发病率越来越高，并且预后严重。2009年有调查显示，我国卵巢肿瘤死亡率居女性生殖道恶性肿瘤之首。众所周知，卵巢肿瘤一个很重要的特征就是容易发生细胞侵袭转移，这种转移特性除了与患者自身原因有关外，卵巢肿瘤干细胞中的趋化因子发挥了重要的作用[1]。已有研究表明多种趋化因子可在卵巢恶性肿瘤中高表达，如近年研究较多且作用较肯定的有白细胞介素8(IL-8)、单核细胞趋化蛋白-1(MCP-1)和巨噬细胞炎性蛋白-1(MIP-1)等。CXCR4(CXC chemokinereceptor 4)是一种趋化因子受体，能在卵巢肿瘤干细胞上特异表达，是当前研究的14个趋化因子受体中唯一在卵巢肿瘤干细胞上表达的；趋化因子CXCL12族(过去又称SDF-1，stromal cell derived factor-1)与其特异性受体CXCR4所构成的复合体在多卵巢肿瘤的分布与侵袭转移中发挥着重要的作用[2]。本研究从体外实验水平探讨卵巢肿瘤干细胞CXCR4表达变化对卵巢肿瘤细胞侵袭转移的影响，希望为卵巢肿瘤的治疗提供新的研究思路和药物作用靶点。
1 资料与方法
1.1 卵巢肿瘤干细胞的培养
本实验所用CAOV-3卵巢细胞株和Anglne细胞株分别购自中国科学院上海细胞所与北京微生物所，将细胞分别接种于含10%胎牛血清标准培养基中，在37℃、5%CO2、饱和湿度环境中培养24 h，确保细胞数不少于1×104个。
1.2 卵巢肿瘤干细胞稳定表达CXCR4体系的建立, 百拇医药(李青 汪艳 张曦 朱慧芬 唐良萏)
[关键词] 卵巢肿瘤干细胞；CXCL12；CXCR4；CAOV-3；侵袭转移
[中图分类号] R737.31 [文献标识码] A [文章编号] 1674-4721(2012)06(a)-0010-03
The relation of chemokine expression and cell invasion and metastasis in the ovarian cancer stem cells
LI Qing1,2 WANG Yan2 ZHANG Xi2 ZHU Huifen2 TANG Liangdan1
1.Department of Obstertrics and Gynecology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; 2.Department of Obstertrics and Gynecology, the Affiliated Anqing Hospital of Anhui Medical University, Anqing 246000, China
[Abstract] Objective To investigate the ovarian tumor stem cells chemokine expression and cell invasion and metastasis ability of relation, for the treatment of ovarian tumors to provide new research ideas and targets of drug action. Methods Expression of CXCR4 in ovarian cancer cellline CAOV-3 was detected by reverse transcriptase—polymerase chain reaction(RT-PCR) and flow cytometry; Transwell was used to analyze the invasion and metastasis of CAOV-3 cells in presence of CXCL12 or when CXCR4 was blocked by anti-CXCR4. Results CXCR4 in CAOV-3 cells showed positive expression, CXCL12 induced the invasion and metastasis of CAOV-3 cells，which could be effectively blocked by anti-CXCR4. Conclusion CXCL12-CXCR4 interactions may promote ovarian tumor stem cells invasion and metastasis, in ovarian tumor growth and metastasis plays an important role in the process.
[Key words] Ovarian cancer stem cells; CXCL12; CXCR4; CAOV-3; Invasion and metastasis
随着社会经济的发展与环境的各种影响，当前我国卵巢肿瘤发病率越来越高，并且预后严重。2009年有调查显示，我国卵巢肿瘤死亡率居女性生殖道恶性肿瘤之首。众所周知，卵巢肿瘤一个很重要的特征就是容易发生细胞侵袭转移，这种转移特性除了与患者自身原因有关外，卵巢肿瘤干细胞中的趋化因子发挥了重要的作用[1]。已有研究表明多种趋化因子可在卵巢恶性肿瘤中高表达，如近年研究较多且作用较肯定的有白细胞介素8(IL-8)、单核细胞趋化蛋白-1(MCP-1)和巨噬细胞炎性蛋白-1(MIP-1)等。CXCR4(CXC chemokinereceptor 4)是一种趋化因子受体，能在卵巢肿瘤干细胞上特异表达，是当前研究的14个趋化因子受体中唯一在卵巢肿瘤干细胞上表达的；趋化因子CXCL12族(过去又称SDF-1，stromal cell derived factor-1)与其特异性受体CXCR4所构成的复合体在多卵巢肿瘤的分布与侵袭转移中发挥着重要的作用[2]。本研究从体外实验水平探讨卵巢肿瘤干细胞CXCR4表达变化对卵巢肿瘤细胞侵袭转移的影响，希望为卵巢肿瘤的治疗提供新的研究思路和药物作用靶点。
1 资料与方法
1.1 卵巢肿瘤干细胞的培养
本实验所用CAOV-3卵巢细胞株和Anglne细胞株分别购自中国科学院上海细胞所与北京微生物所，将细胞分别接种于含10%胎牛血清标准培养基中，在37℃、5%CO2、饱和湿度环境中培养24 h，确保细胞数不少于1×104个。
1.2 卵巢肿瘤干细胞稳定表达CXCR4体系的建立, 百拇医药(李青 汪艳 张曦 朱慧芬 唐良萏)